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Production, crystallization and structure determination of a mycobacterial glucosylglycerate hydrolase
Author(s) -
Cereija Tatiana Barros,
Alarico Susana,
Empadinhas Nuno,
Pereira Pedro José Barbosa
Publication year - 2017
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 37
ISSN - 2053-230X
DOI - 10.1107/s2053230x17012419
Subject(s) - crystallization , monoclinic crystal system , hydrolase , diffraction , resolution (logic) , solvent , crystallography , synchrotron radiation , chemistry , materials science , crystal structure , enzyme , optics , physics , biochemistry , organic chemistry , artificial intelligence , computer science
Glucosylglycerate hydrolase is highly conserved among rapidly growing mycobacteria and has been found to be involved in recovery from nitrogen starvation by promoting the rapid mobilization of the glucosylglycerate that accumulates under these conditions. Here, the production, crystallization and structure determination of glucosylglycerate hydrolase from Mycobacterium hassiacum using two‐wavelength anomalous diffraction of selenomethionine‐substituted crystals are described. The monoclinic (space group P 2 1 ) crystals diffracted to ∼2.0 Å resolution at a synchrotron‐radiation source and contained four molecules in the asymmetric unit, corresponding to a Matthews coefficient of 3.07 Å 3 Da −1 and a solvent content of 59.9%. The quality of the experimental phases allowed the automated building of 1677 of the 1792 residues in the asymmetric unit.