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Transcription factor Rv0081 from Mycobacterium tuberculosis : purification, crystallization and initial crystallographic analysis
Author(s) -
Dong Shishang,
Ding Zhenzhen,
Wang Yu,
Yang Yan,
Mao Yonghong,
Wang Ying
Publication year - 2017
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 37
ISSN - 2053-230X
DOI - 10.1107/s2053230x17005064
Subject(s) - crystallization , mycobacterium tuberculosis , tuberculosis , transcription factor , crystallography , infectivity , transcription (linguistics) , chemistry , crystal structure , pathogenicity , biology , microbiology and biotechnology , genetics , gene , medicine , pathology , linguistics , virus , philosophy , organic chemistry
Because of its high infectivity and pathogenicity, Mycobacterium tuberculosis is a serious threat to human health. While the transcription‐regulatory system of M. tuberculosis remains incompletely understood, Rv0081, an essential regulatory hub, is known to mediate the initial response to hypoxia in the long‐term survival of M. tuberculosis . Here, the production, crystallization and initial X‐ray crystallographic analysis of Rv0081 are reported. The crystals of Rv0081 belonged to space group P 6 2 , with unit‐cell parameters a = 67.48, b = 67.48, c = 40.84 Å, γ = 120°. The Matthews coefficient is 2.09 Å 3 Da −1 , assuming the presence of one molecule in the asymmetric unit, with a corresponding solvent content of 41.27%. Phasing of the native crystal form of Rv0081 was performed by molecular replacement. Currently, the structure has been refined to 2.00 Å resolution with an R work of 25.99% and an R free of 30.88%.