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Crystal structure of d(CCGGGGTACCCCGG) 2 at 1.4 Å resolution
Author(s) -
Karthik Selvam,
Thirugnanasambandam Arunachalam,
Mandal Pradeep Kumar,
Gautham Namasivayam
Publication year - 2017
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 37
ISSN - 2053-230X
DOI - 10.1107/s2053230x17004770
Subject(s) - tetragonal crystal system , crystallography , crystal structure , resolution (logic) , duplex (building) , molecule , helix (gastropod) , sequence (biology) , crystal (programming language) , chemistry , dna , stereochemistry , biology , ecology , biochemistry , organic chemistry , artificial intelligence , snail , computer science , programming language
The X‐ray crystal structure of the DNA tetradecamer sequence d(CCGGGGTACCCCGG) 2 is reported at 1.4 Å resolution in the tetragonal space group P 4 1 2 1 2. The sequence was designed to fold as a four‐way junction. However, it forms an A‐type double helix in the presence of barium chloride. The metal ion could not be identified in the electron‐density map. The crystallographic asymmetric unit consists of one A‐type double helix with 12 base pairs per turn, in contrast to 11 base pairs per turn for canonical A‐DNA. A large number of solvent molecules have been identified in both the grooves of the duplex and around the backbone phosphate groups.