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Crystallization and preliminary X‐ray diffraction studies of frutalin, an α‐D‐galactose‐specific lectin from Artocarpus incisa seeds
Author(s) -
MonteiroMoreira Ana Cristina de Oliveira,
D'Muniz Pereira Humberto,
Vieira Neto Antonio Eufrasio,
Mendes Batista Moreno Frederico Bruno,
Duarte Pinto Lobo Marina,
Sousa Felipe Domingos de,
Moreira Renato de Azevedo
Publication year - 2015
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 37
ISSN - 2053-230X
DOI - 10.1107/s2053230x15015186
Subject(s) - crystallization , monoclinic crystal system , chemistry , lectin , crystallography , monomer , citric acid , artocarpus , galactose , x ray crystallography , diffraction , crystal structure , biochemistry , polymer , organic chemistry , biology , botany , physics , optics
Frutalin is an α‐D‐galactose‐specific carbohydrate‐binding glycoprotein with antitumour properties and is a powerful tool for tumour biomarker discovery. The crystallization and preliminary X‐ray diffraction analysis of this lectin, which was isolated from Artocarpus incisa seeds, are reported here. Frutalin was purified and submitted to mass‐spectrometric analysis. Diverse masses at approximately 16 kDa were observed in the deconvoluted spectra, which support the presence of isoforms. The best frutalin crystals were grown within a week in 0.1 M citric acid pH 3.5 which contained 25% PEG 3350 as a precipitant at 293 K, and diffracted to a maximum resolution of 1.81 Å. The monoclinic crystals belonged to space group I 2, with unit‐cell parameters a = 76.17, b = 74.56, c = 118.98 Å, β = 96.56°. A molecular‐replacement solution was obtained which indicated the presence of four monomers per asymmetric unit. Crystallographic refinement of the structure is in progress.