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Analysis of crystallization data in the Protein Data Bank
Author(s) -
Kirkwood Jobie,
Hargreaves David,
O'Keefe Simon,
Wilson Julie
Publication year - 2015
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 37
ISSN - 2053-230X
DOI - 10.1107/s2053230x15014892
Subject(s) - protein data bank , protein data bank (rcsb pdb) , crystallization , isoelectric point , protein crystallization , computer science , chemistry , protein structure , data mining , crystallography , biochemistry , organic chemistry , enzyme
The Protein Data Bank (PDB) is the largest available repository of solved protein structures and contains a wealth of information on successful crystallization. Many centres have used their own experimental data to draw conclusions about proteins and the conditions in which they crystallize. Here, data from the PDB were used to reanalyse some of these results. The most successful crystallization reagents were identified, the link between solution pH and the isoelectric point of the protein was investigated and the possibility of predicting whether a protein will crystallize was explored.