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Cloning, expression, purification and crystallographic studies of galectin‐11 from domestic sheep ( Ovis aries )
Author(s) -
Sakthivel Dhanasekaran,
Littler Dene,
Shahine Adam,
Troy Sally,
Johnson Matthew,
Rossjohn Jamie,
Piedrafita David,
Beddoe Travis
Publication year - 2015
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 37
ISSN - 2053-230X
DOI - 10.1107/s2053230x15010195
Subject(s) - galectin , ovis , biology , cloning (programming) , glycan , evolutionary biology , computational biology , innate immune system , livestock , microbiology and biotechnology , genetics , immune system , glycoprotein , ecology , computer science , programming language
Galectins are an evolutionarily conserved family of proteins that translate glycan recognition into cellular effects. Galectin‐11 is a unique member of the galectin family that is only expressed in ruminants such as sheep, goat and cattle and that plays a critical role in several important biological processes, such as reproduction and parasite‐mediated innate immune responses. Currently, these two areas are of major importance for the sustainability of ruminant livestock production. Despite the emerging biological significance of galectin‐11, no structural information is available. It is expected that structural studies will unravel the functional mechanisms of galectin‐11 activity. Here, the expression, purification and crystallization of the ruminant‐specific galectin‐11 from domestic sheep and the collection of X‐ray data to 2.0 Å resolution are reported.