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Molecular cloning, overexpression, purification and crystallographic analysis of a GH43 β‐xylosidase from Bacillus licheniformis
Author(s) -
Diogo José Alberto,
Zanphorlin Leticia Maria,
Sato Hélia Harumi,
Murakami Mario Tyago,
Ruller Roberto
Publication year - 2015
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 37
ISSN - 2053-230X
DOI - 10.1107/s2053230x15009978
Subject(s) - bacillus licheniformis , biochemistry , escherichia coli , xylose , chemistry , hemicellulose , xylanase , bacterial cellulose , hydrolysis , enzyme , biology , cellulose , bacteria , gene , fermentation , bacillus subtilis , genetics
β‐Xylosidases (EC 3.2.1.37) catalyze the hydrolysis of short xylooligosaccharides into xylose, which is an essential step in the complete depolymerization of xylan, the major hemicellulosic polysaccharide of plant cell walls, and has great biotechnological relevance for the production of lignocellulose‐based biofuels and the paper industry. In this study, a GH43 β‐xylosidase identified from the bacterium Bacillus licheniformis (BlXylA) was cloned into the the pET‐28a bacterial expression vector, recombinantly overexpressed in Escherichia coli BL21(DE3) cells and purified to homogeneity by metal‐affinity and size‐exclusion chromatography. The protein was crystallized in the presence of the organic solvent 2‐methyl‐2,4‐pentanediol and a single crystal diffracted to 2.49 Å resolution. The X‐ray diffraction data were indexed in the monoclinic space group C 2, with unit‐cell parameters a = 152.82, b = 41.9, c = 71.79 Å, β = 91.7°. Structural characterization of this enzyme will contribute to a better understanding of the structural requirements for xylooligosaccharide specificity within the GH43 family.