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Crystallographic studies of the extracytoplasmic function σ factor σ J from Mycobacterium tuberculosis
Author(s) -
Goutam Kapil,
Gupta Arvind Kumar,
Gopal Balasubramanian
Publication year - 2015
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 37
ISSN - 2053-230X
DOI - 10.1107/s2053230x15009577
Subject(s) - tetragonal crystal system , mycobacterium tuberculosis , synchrotron radiation , microbiology and biotechnology , hydrogen peroxide , tuberculosis , bacteria , beamline , crystallography , chemistry , biology , physics , crystal structure , genetics , biochemistry , optics , medicine , pathology , beam (structure)
Mycobacterium tuberculosis has multiple σ factors which enable the bacterium to reprogram its transcriptional machinery under diverse environmental conditions. σ J , an extracytoplasmic function σ factor, is upregulated in late stationary phase cultures and during human macrophage infection. σ J governs the cellular response to hydrogen peroxide‐mediated oxidative stress. σ J differs from other canonical σ factors owing to the presence of a SnoaL_2 domain at the C‐terminus. σ J crystals belonged to the tetragonal space group I 422, with unit‐cell parameters a = b = 133.85, c = 75.08 Å. Diffraction data were collected to 2.16 Å resolution on the BM14 beamline at the European Synchrotron Radiation Facility (ESRF).