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Cloning, expression, purification, crystallization and preliminary X‐ray diffraction studies of NAD synthetase from methicillin‐resistant Staphylococcus aureus
Author(s) -
Arbade Gajanan Kashinathrao,
Srivastava Sandeep Kumar
Publication year - 2015
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 37
ISSN - 2053-230X
DOI - 10.1107/s2053230x15007906
Subject(s) - nad+ kinase , cofactor , purine , nicotinamide adenine dinucleotide , staphylococcus aureus , enzyme , biochemistry , biosynthesis , biology , microbiology and biotechnology , context (archaeology) , chemistry , bacteria , genetics , paleontology
Staphylococcus aureus is an important human and animal pathogen that causes a wide range of infections. The prevalence of multidrug‐resistant S. aureus strains in both hospital and community settings makes it imperative to characterize new drug targets to combat S. aureus infections. In this context, enzymes involved in NAD metabolism and synthesis are significant drug targets as NAD is a central player in several cellular processes. NAD synthetase catalyzes the last step in the biosynthesis of nicotinamide adenine dinucleotide, making it a crucial intermediate enzyme linked to the biosynthesis of several amino acids, purine and pyrimidine nucleotides, coenzymes and antibiotics.