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Purification, crystallization and X‐ray crystallographic studies of a Bacillus cereus MepR‐like transcription factor, BC0657
Author(s) -
Cho Min Uk,
Kim Meong Il,
Hong Minsun
Publication year - 2015
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 37
ISSN - 2053-230X
DOI - 10.1107/s2053230x15007074
Subject(s) - bacillus cereus , transcription (linguistics) , transcription factor , staphylococcus aureus , cereus , regulator , biology , crystallization , transcriptional regulation , multiple drug resistance , antibiotic resistance , microbiology and biotechnology , genetics , crystallography , bacteria , chemistry , antibiotics , gene , linguistics , organic chemistry , philosophy
Transcription factors of the MarR family respond to internal and external changes and regulate a variety of biological functions through ligand association with microorganisms. MepR belongs to the MarR family, and its mutations are associated with the development of multidrug resistance in Staphylococcus aureus , which has caused a growing health problem. In this study, a Bacillus cereus MepR‐like transcription regulator, BC0657, was crystallized. The BC0657 crystals diffracted to 2.05 Å resolution and belonged to either space group P 6 2 22 or P 6 4 22, with unit‐cell parameters a = 110.57, b = 110.57, c = 67.29 Å. There was one molecule per asymmetric unit. Future comparative structural studies on BC0657 would extend knowledge of ligand‐induced transcriptional regulatory mechanisms in the MarR family and would make a significant contribution to the design of antibiotic drugs against multidrug‐resistant bacteria.