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Structures of prostaglandin F synthase from the protozoa Leishmania major and Trypanosoma cruzi with NADP
Author(s) -
Moen Spencer O.,
Fairman James W.,
Barnes Steve R.,
Sullivan Amy,
NakazawaHewitt Stephen,
Van Voorhis Wesley C.,
Staker Bart L.,
Lorimer Donald D.,
Myler Peter J.,
Edwards Thomas E.
Publication year - 2015
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 37
ISSN - 2053-230X
DOI - 10.1107/s2053230x15006883
Subject(s) - trypanosoma cruzi , protozoa , atp synthase , trypanosoma , leishmania , cofactor , biology , chagas disease , chemistry , biochemistry , microbiology and biotechnology , enzyme , virology , parasite hosting , world wide web , computer science
The crystal structures of prostaglandin F synthase (PGF) from both Leishmania major and Trypanosoma cruzi with and without their cofactor NADP have been determined to resolutions of 2.6 Å for T. cruzi PGF, 1.25 Å for T. cruzi PGF with NADP, 1.6 Å for L. major PGF and 1.8 Å for L. major PGF with NADP. These structures were determined by molecular replacement to a final R factor of less than 18.6% ( R free of less than 22.9%). PGF in the infectious protozoa L. major and T. cruzi is a potential therapeutic target.