Expression, purification and crystallization of a family 55 β‐1,3‐glucanase from  Chaetomium thermophilum | Zendy

Papageorgiou Anastassios C. | Zendy; Li Duochuan | Zendy

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Expression, purification and crystallization of a family 55 β‐1,3‐glucanase from Chaetomium thermophilum

Author(s) -

Papageorgiou Anastassios C.,

Li Duochuan

Publication year - 2015

Publication title -

acta crystallographica section f

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.572

H-Index - 37

ISSN - 2053-230X

DOI - 10.1107/s2053230x15006366

Subject(s) - pichia pastoris , glucanase , chaetomium , thermophile , crystallization , fungus , sodium , chemistry , crystal (programming language) , recombinant dna , potassium , biology , stereochemistry , botany , biochemistry , food science , enzyme , organic chemistry , gene , computer science , programming language , penicillium

A β‐1,3‐glucanase from the thermophilic fungus Chaetomium thermophilum was overexpressed in Pichia pastoris , purified and crystallized in the presence of 1.8 M sodium/potassium phosphate pH 6.8 as a precipitant. Data to 2.0 Å resolution were collected in‐house at 293 K from a single crystal. The crystal was found to belong to space group P2 1 , with unit‐cell parameters a = 64.1, b = 85.8, c = 68.5 Å, β = 93.1° and one molecule in the asymmetric unit.

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