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Expression, purification and crystallization of a family 55 β‐1,3‐glucanase from Chaetomium thermophilum
Author(s) -
Papageorgiou Anastassios C.,
Li Duochuan
Publication year - 2015
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 37
ISSN - 2053-230X
DOI - 10.1107/s2053230x15006366
Subject(s) - pichia pastoris , glucanase , chaetomium , thermophile , crystallization , fungus , sodium , chemistry , crystal (programming language) , recombinant dna , potassium , biology , stereochemistry , botany , biochemistry , food science , enzyme , organic chemistry , gene , computer science , programming language , penicillium
A β‐1,3‐glucanase from the thermophilic fungus Chaetomium thermophilum was overexpressed in Pichia pastoris , purified and crystallized in the presence of 1.8 M sodium/potassium phosphate pH 6.8 as a precipitant. Data to 2.0 Å resolution were collected in‐house at 293 K from a single crystal. The crystal was found to belong to space group P2 1 , with unit‐cell parameters a = 64.1, b = 85.8, c = 68.5 Å, β = 93.1° and one molecule in the asymmetric unit.