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The three‐dimensional structure of the cellobiohydrolase Cel7A from Aspergillus fumigatus at 1.5 Å resolution
Author(s) -
Moroz Olga V.,
Maranta Michelle,
Shaghasi Tarana,
Harris Paul V.,
Wilson Keith S.,
Davies Gideon J.
Publication year - 2015
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 37
ISSN - 2053-230X
DOI - 10.1107/s2053230x14027307
Subject(s) - aspergillus fumigatus , chemistry , disaccharide , glycoside hydrolase , biocatalysis , enzyme , hydrolase , cellulose , cell wall , stereochemistry , biochemistry , biology , botany , catalysis , reaction mechanism
The enzymatic degradation of plant cell‐wall cellulose is central to many industrial processes, including second‐generation biofuel production. Key players in this deconstruction are the fungal cellobiohydrolases (CBHs), notably those from family GH7 of the carbohydrate‐active enzymes (CAZY) database, which are generally known as CBHI enzymes. Here, three‐dimensional structures are reported of the Aspergillus fumigatus CBHI Cel7A solved in uncomplexed and disaccharide‐bound forms at resolutions of 1.8 and 1.5 Å, respectively. The product complex with a disaccharide in the +1 and +2 subsites adds to the growing three‐dimensional insight into this family of industrially relevant biocatalysts.