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Overexpression, purification, crystallization and preliminary X‐ray crystallographic analysis of SF173 from Shigella flexneri
Author(s) -
Kim HaNeul,
An JeongGi,
Lee YooSup,
Seok SeungHyeon,
Yoo HeeSeop,
Seo MinDuk
Publication year - 2015
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 37
ISSN - 2053-230X
DOI - 10.1107/s2053230x14025941
Subject(s) - shigella flexneri , crystallization , crystallography , structural genomics , shigella , microbiology and biotechnology , bacteria , chemistry , biology , escherichia coli , biochemistry , protein structure , gene , genetics , organic chemistry
Shigella flexneri is a Gram‐negative, anaerobic bacterium in the genus Shigella that can cause diarrhoea in humans. SF173, a hypothetical protein from S. flexneri 5a strain M90T, has been cloned, overexpressed, purified and crystallized as a part of laboratory‐scale structural genomics project. The SF173 protein was crystallized using the sitting‐drop vapour‐diffusion method in the presence of 0.8 M succinic acid pH 7.0 at 293 K. Preliminary X‐ray diffraction analysis revealed that the crystal diffracted to 1.47 Å resolution and belonged to space group I 432, with unit‐cell parameters a = b = c = 110.245 Å.
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