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Crystallization and preliminary crystallographic analysis of poly(3‐hydroxybutyrate) depolymerase from Bacillus thuringiensis
Author(s) -
Wang YungLin,
Lin YiTing,
Chen ChiaLin,
Shaw GwoChyuan,
Liaw ShwuHuey
Publication year - 2014
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 37
ISSN - 2053-230X
DOI - 10.1107/s2053230x14019347
Subject(s) - crystallization , bacillus thuringiensis , polyethylene glycol , biopolymer , polybutylene succinate , materials science , chemistry , polymer , polymer chemistry , bacteria , organic chemistry , biology , genetics
Poly[( R )‐3‐hydroxybutyrate] (PHB) is a microbial biopolymer that has been commercialized as biodegradable plastics. The key enzyme for the degradation is PHB depolymerase (PhaZ). A new intracellular PhaZ from Bacillus thuringiensis (BtPhaZ) has been screened for potential applications in polymer biodegradation. Recombinant BtPhaZ was crystallized using 25% polyethylene glycol 3350, 0.2 M ammonium acetate, 0.1 M bis‐tris pH 6.5 at 288 K. The crystals belonged to space group P 1, with unit‐cell parameters a = 42.97, b = 83.23, c = 85.50 Å, α = 73.45, β = 82.83, γ = 83.49°. An X‐ray diffraction data set was collected to 1.42 Å resolution with an R merge of 6.4%. Unexpectedly, a molecular‐replacement solution was obtained using the crystal structure of Streptomyces lividans chloroperoxidase as a template, which shares 24% sequence identity to BtPhaZ. This is the first crystal structure of an intracellular poly(3‐hydroxybutyrate) depolymerase.