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Complex assembly, crystallization and preliminary X‐ray crystallographic analysis of the chicken CD8αα–BF2*0401 complex
Author(s) -
Liu Yanjie,
Chen Rong,
Tariq Mansoor,
Xia Chun
Publication year - 2014
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 37
ISSN - 2053-230X
DOI - 10.1107/s2053230x14017154
Subject(s) - crystallization , crystallography , crystal structure , cd8 , beta 2 microglobulin , chemistry , mhc class i , major histocompatibility complex , crystal (programming language) , cytotoxic t cell , antigen , biology , biochemistry , immunology , gene , computer science , organic chemistry , programming language , in vitro
In the process of antigen presentation, the MHCI–CD8 complex is important for immune signal transduction by the activation of cytotoxic T cells. Here, the expression, purification, crystallization and X‐ray analysis of the complex of the chicken MHC class I molecule BF2*0401 and CD8αα (CD8αα–BF2*0401) are reported. This complex was verified by SDS–PAGE analysis of a CD8αα–BF2*0401 crystal, which showed three bands corresponding to the molecular weights of BF2*0401, β 2 ‐microglobulin and CD8α, respectively. The crystal of CD8αα–BF2*0401 diffracted to 2.8 Å resolution and belonged to space group P 2 1 , with unit‐cell parameters a = 90.6, b = 90.8, c = 94.9 Å, β = 98°. The Matthews coefficient and solvent content were calculated to be 2.88 Å 3 Da −1 and ∼57.3%, respectively.