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Cloning, expression, purification, crystallization and preliminary crystallographic analysis of the C‐terminal domain of Par‐4 (PAWR)
Author(s) -
Tiruttani Subhramanyam Udaya Kumar,
Kubicek Jan,
Eidhoff Ulf B.,
Labahn Joerg
Publication year - 2014
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 37
ISSN - 2053-230X
DOI - 10.1107/s2053230x14014691
Subject(s) - cloning (programming) , crystallization , terminal (telecommunication) , crystallography , domain (mathematical analysis) , expression (computer science) , chemistry , microbiology and biotechnology , computational biology , materials science , biology , computer science , mathematics , organic chemistry , programming language , telecommunications , mathematical analysis
Prostate apoptosis response‐4 protein is an intrinsically disordered pro‐apoptotic protein with tumour suppressor function. Par‐4 is known for its selective induction of apoptosis in cancer cells only and its ability to interact with various apoptotic proteins via its C‐terminus. Par‐4, with its unique function and various interacting partners, has gained importance as a potential target for cancer therapy. The C‐terminus of the rat homologue of Par‐4 was crystallized and a 3.7 Å resolution X‐ray diffraction data set was collected. Preliminary data analysis shows the space group to be P 4 1 2 1 2. The unit‐cell parameters are a = b = 115.351, c = 123.663 Å, α = β = γ = 90°.