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Expression, purification, crystallization and preliminary X‐ray analysis of the HER3–9E12 Fab complex
Author(s) -
He Kecheng,
Huang Ang,
Huang Yong,
Takeda Hiroaki
Publication year - 2014
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 37
ISSN - 2053-230X
DOI - 10.1107/s2053230x14008917
Subject(s) - antibody , recombinant dna , monoclonal antibody , immunoglobulin fab fragments , epidermal growth factor receptor , microbiology and biotechnology , receptor , biology , computational biology , chemistry , immunology , biochemistry , gene , complementarity determining region
9E12 is a fully human immunoglobulin G 1 /κ monoclonal antibody that is specific for the epidermal growth factor receptor 3 (HER3), the overexpression of which has been detected in many tumour types and is associated with poor survival outcomes. To date, knowledge of the molecular mechanism for targeted antibodies that directly inhibit HER3 signalling is limited. Because knowledge of such therapeutic antibodies would help basic immunological therapeutics, structural insights into the HER3–9E12 Fab complex are important. Recombinant human HER3 and Fab fragments of the 9E12 antibody were cloned, expressed and crystallized, and crystallographic data sets were collected. The crystals belonged to space group P 1, with unit‐cell parameters a = 74.4, b = 98.6, c = 99.6 Å, α = 106.0, β = 95.0, γ = 102.5° and diffracted to a resolution of 2.1 Å.