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Crystallization and preliminary X‐ray study of TsiV3 from Vibrio cholerae
Author(s) -
Zhang Jiulong,
Zhang Heng,
Liu Ying,
Zhan Lihong,
She Zhun,
Dong Cheng,
Dong Yuhui
Publication year - 2014
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 37
ISSN - 2053-230X
DOI - 10.1107/s2053230x14001599
Subject(s) - vibrio cholerae , crystallization , microbiology and biotechnology , biology , physics , bacteria , thermodynamics , genetics
The bacterial type VI secretion system (T6SS), a dynamic organelle, participates in microbial competition by transporting toxic effector molecules to neighbouring cells to kill competitors. TsiV3, a recently defined T6SS immunity protein in Vibrio cholerae , possesses self‐protection against killing by T6SS predatory cells by directly binding to and inhibiting their effector protein VgrG‐3. Structural information about TsiV3 could help to illuminate its specific mechanism. In this study, TsiV3 from V. cholerae was cloned, expressed and crystallized and single‐crystal X‐ray diffraction data sets were collected to a resolution of 2.55 Å. Specifically, the crystal belonged to space group P 2 1 2 1 2 1 , with unit‐cell parameters a = 73.3, b = 78.12, c = 106.18 Å. Matthews coefficient calculations indicated that the crystal may contain six TsiV3 molecules in one asymmetric unit, with a V M value of 2.25 Å 3 Da −1 and a solvent content of 45.42%.