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Crystallization and preliminary X‐ray characterization of MutT2, MSMEG_5148 from Mycobacterium smegmatis
Author(s) -
Arif S. M.,
Sang P. B.,
Varshney U.,
Vijayan M.
Publication year - 2014
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 37
ISSN - 2053-230X
DOI - 10.1107/s2053230x13033906
Subject(s) - mycobacterium smegmatis , orthorhombic crystal system , crystallization , crystallography , hydrolase , chemistry , crystal structure , biochemistry , enzyme , mycobacterium tuberculosis , organic chemistry , medicine , tuberculosis , pathology
Crystallization of MutT2, MSMEG_5148 from Mycobacterium smegmatis , has been carried out and the crystals have been characterized using X‐ray diffraction. Matthews coefficient calculation suggests the possibility of one protein molecule in the asymmetric unit of the orthorhombic unit cell, space group P 2 1 2 1 2 or P 2 1 22. Solution of the structure of the protein by molecular replacement using the known three‐dimensional structure of a bacterial Nudix hydrolase is envisaged.