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Purification, crystallization and preliminary X‐ray diffraction analysis of a novel keto‐deoxy‐D‐galactarate (KDG) dehydratase from Agrobacterium tumefaciens
Author(s) -
Taberman Helena,
Andberg Martina,
Parkkinen Tarja,
Richard Peter,
Hakulinen Nina,
Koivula Anu,
Rouvinen Juha
Publication year - 2014
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 37
ISSN - 2053-230X
DOI - 10.1107/s2053230x13031361
Subject(s) - agrobacterium tumefaciens , crystallization , dehydratase , chemistry , crystallography , biochemistry , transformation (genetics) , enzyme , gene , organic chemistry
D‐Galacturonic acid is the main component of pectin. It could be used to produce affordable renewable fuels, chemicals and materials through biotechnical conversion. Keto‐deoxy‐D‐galactarate (KDG) dehydratase is an enzyme in the oxidative pathway of D‐galacturonic acid in Agrobacterium tumefaciens ( At ). It converts 3‐deoxy‐2‐keto‐L‐ threo ‐hexarate to α‐ketoglutaric semialdehyde. At KDG dehydratase was crystallized by the hanging‐drop vapour‐diffusion method. The crystals belonged to the monoclinic space group C 2, with unit‐cell parameters a = 169.1, b = 117.8, c = 74.3 Å, β = 112.4° and an asymmetric unit of four monomers. X‐ray diffraction data were collected to 1.9 Å resolution using synchrotron radiation. The three‐dimensional structure of At KDG dehydratase will provide valuable information on the function of the enzyme and will allow it to be engineered for biorefinery‐based applications.