Open AccessQuantitative phase measurements of human cell nuclei using X‐ray ptychography
Author(s) -
Schwenke Jorg,
Yusuf Mohammed,
Shemilt Laura A.,
Wagner Ulrich,
Sajid Atiqa,
Morrison Graeme R.,
Zhang Fucai,
Parsons Aaron,
Rau Christoph,
Robinson Ian K.
Publication year - 2021
Publication title -
journal of synchrotron radiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.172
H-Index - 99
ISSN - 1600-5775
DOI - 10.1107/s1600577521004586
Subject(s) - ptychography , optics , physics , beamline , coherence (philosophical gambling strategy) , phase (matter) , phase retrieval , scattering , diffraction , fourier transform , beam (structure) , quantum mechanics
The human cell nucleus serves as an important organelle holding the genetic blueprint for life. In this work, X‐ray ptychography was applied to assess the masses of human cell nuclei using its unique phase shift information. Measurements were carried out at the I13‐1 beamline at the Diamond Light Source that has extremely large transverse coherence properties. The ptychographic diffractive imaging approach allowed imaging of large structures that gave quantitative measurements of the phase shift in 2D projections. In this paper a modified ptychography algorithm that improves the quality of the reconstruction for weak scattering samples is presented. The application of this approach to calculate the mass of several human nuclei is also demonstrated.