Open AccessUpgrade of BL‐5C as a highly automated macromolecular crystallography beamline at Pohang Light Source II
Author(s) -
Jeong Jae-Hee,
Eo Cheolsoo,
Kim Hyo-Yun,
Kim Jin-Hong,
Lee Chae-Soon,
Choi Hyeong-Joo,
Kim Yeon-Gil
Publication year - 2021
Publication title -
journal of synchrotron radiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.172
H-Index - 99
ISSN - 1600-5775
DOI - 10.1107/s1600577521000588
Subject(s) - beamline , undulator , optics , microbeam , physics , diffractometer , aperture (computer memory) , detector , beam (structure) , scanning electron microscope , acoustics
BL‐5C is an in‐vacuum undulator beamline dedicated to macromolecular crystallography (MX) at the 3 GeV Pohang Light Source II in Korea. The beamline delivers X‐ray beams with a focal spot size of 200 µm × 40 µm (FWHM, H × V) over the energy range 6.5–16.5 keV. The measured flux is 7 × 10 11 photons s −1 at 12.659 keV through an aperture size of 50 µm. The experimental station is newly equipped with the photon‐counting detector EIGER 9M, the multi‐axis micro‐diffractometer MD2, and a robotic sample changer with a high‐capacity dewar. These instruments enable the operation of this beamline as an automated MX beamline specialized in X‐ray fragment screening. This beamline can collect more than 400 data sets a day without human intervention, and a difference map can be automatically calculated by using the data processing pipeline for ligand or fragment identification.