Open AccessUse of continuous sample translation to reduce radiation damage for XPCS studies of protein diffusion
Author(s) -
Lurio Laurence B.,
Thurston George M.,
Zhang Qingteng,
Narayanan Suresh,
Dufresne Eric M.
Publication year - 2021
Publication title -
journal of synchrotron radiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.172
H-Index - 99
ISSN - 1600-5775
DOI - 10.1107/s1600577521000035
Subject(s) - synchrotron radiation , sample (material) , synchrotron , diffusion , optics , beam (structure) , microsecond , translation (biology) , radiation damage , physics , noise (video) , materials science , chemistry , computer science , radiation , messenger rna , artificial intelligence , biochemistry , gene , thermodynamics , image (mathematics)
An experimental setup to measure X‐ray photon correlation spectroscopy during continuous sample translation is presented and its effectiveness as a means to avoid sample damage in dynamics studies of protein diffusion is evaluated. X‐ray damage from focused coherent synchrotron radiation remains below tolerable levels as long as the sample is translated through the beam sufficiently quickly. Here it is shown that it is possible to separate sample dynamics from the effects associated with the transit of the sample through the beam. By varying the sample translation rate, the damage threshold level, D thresh = 1.8 kGy, for when beam damage begins to modify the dynamics under the conditions used, is also determined. Signal‐to‐noise ratios, R sn ≥ 20, are obtained down to the shortest delay times of 20 µs. The applicability of this method of data collection to the next generation of multi‐bend achromat synchrotron sources is discussed and it is shown that sub‐microsecond dynamics should be obtainable on protein samples.