Open AccessSoft X‐ray induced radiation damage in thin freeze‐dried brain samples studied by FTIR microscopy
Author(s) -
Surowka Artur D.,
Giacelli A.,
Birarda G.,
Sala S.,
Cefarin N.,
Matruglio A.,
Szczerbowska-Boruchowska M.,
Ziomber-Lisiak A.,
Vaccari L.
Publication year - 2020
Publication title -
journal of synchrotron radiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.172
H-Index - 99
ISSN - 1600-5775
DOI - 10.1107/s1600577520010103
Subject(s) - fourier transform infrared spectroscopy , infrared microscopy , microscopy , synchrotron radiation , infrared , materials science , x ray , radiation , radiation damage , nanoscopic scale , synchrotron , membrane , irradiation , resolution (logic) , fourier transform , analytical chemistry (journal) , optics , nanotechnology , chemistry , physics , chromatography , computer science , biochemistry , quantum mechanics , artificial intelligence , nuclear physics
In order to push the spatial resolution limits to the nanoscale, synchrotron‐based soft X‐ray microscopy (XRM) experiments require higher radiation doses to be delivered to materials. Nevertheless, the associated radiation damage impacts on the integrity of delicate biological samples. Herein, the extent of soft X‐ray radiation damage in popular thin freeze‐dried brain tissue samples mounted onto Si 3 N 4 membranes, as highlighted by Fourier transform infrared microscopy (FTIR), is reported. The freeze‐dried tissue samples were found to be affected by general degradation of the vibrational architecture, though these effects were weaker than those observed in paraffin‐embedded and hydrated systems reported in the literature. In addition, weak, reversible and specific features of the tissue–Si 3 N 4 interaction could be identified for the first time upon routine soft X‐ray exposures, further highlighting the complex interplay between the biological sample, its preparation protocol and X‐ray probe.