Open AccessMethods for dynamic synchrotron X‐ray respiratory imaging in live animals
Author(s) -
Morgan Kaye Susannah,
Parsons David,
Cmielewski Patricia,
McCarron Alexandra,
Gradl Regine,
Farrow Nigel,
Siu Karen,
Takeuchi Akihisa,
Suzuki Yoshio,
Uesugi Kentaro,
Uesugi Masayuki,
Yagi Naoto,
Hall Chris,
Klein Mitzi,
Maksimenko Anton,
Stevenson Andrew,
Hausermann Daniel,
Dierolf Martin,
Pfeiffer Franz,
Donnelley Martin
Publication year - 2020
Publication title -
journal of synchrotron radiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.172
H-Index - 99
ISSN - 1600-5775
DOI - 10.1107/s1600577519014863
Subject(s) - synchrotron radiation , synchrotron , medical physics , computer science , optics , physics , nuclear medicine , medicine
Small‐animal physiology studies are typically complicated, but the level of complexity is greatly increased when performing live‐animal X‐ray imaging studies at synchrotron and compact light sources. This group has extensive experience in these types of studies at the SPring‐8 and Australian synchrotrons, as well as the Munich Compact Light Source. These experimental settings produce unique challenges. Experiments are always performed in an isolated radiation enclosure not specifically designed for live‐animal imaging. This requires equipment adapted to physiological monitoring and test‐substance delivery, as well as shuttering to reduce the radiation dose. Experiment designs must also take into account the fixed location, size and orientation of the X‐ray beam. This article describes the techniques developed to overcome the challenges involved in respiratory X‐ray imaging of live animals at synchrotrons, now enabling increasingly sophisticated imaging protocols.