Open AccessReducing sample consumption for serial crystallography using acoustic drop ejection
Author(s) -
Davy Bradley,
Axford Danny,
Beale John H.,
Butryn Agata,
Docker Peter,
Ebrahim Ali,
Leen Gabriel,
Orville Allen M.,
Owen Robin L.,
Aller Pierre
Publication year - 2019
Publication title -
journal of synchrotron radiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.172
H-Index - 99
ISSN - 1600-5775
DOI - 10.1107/s1600577519009329
Subject(s) - drop (telecommunication) , diffraction , sample (material) , materials science , serial communication , laser , cathode ray , slurry , x ray crystallography , beam (structure) , computer science , optics , computer hardware , electron , chemistry , physics , telecommunications , composite material , chromatography , quantum mechanics
Efficient sample delivery is an essential aspect of serial crystallography at both synchrotrons and X‐ray free‐electron lasers. Rastering fixed target chips through the X‐ray beam is an efficient method for serial delivery from the perspectives of both sample consumption and beam time usage. Here, an approach for loading fixed targets using acoustic drop ejection is presented that does not compromise crystal quality, can reduce sample consumption by more than an order of magnitude and allows serial diffraction to be collected from a larger proportion of the crystals in the slurry.