Open AccessXANES and EXAFS of dilute solutions of transition metals at XFELs
Author(s) -
Chatterjee Ruchira,
Weninger Clemens,
Loukianov Anton,
Gul Sheraz,
Fuller Franklin D.,
Cheah Mun Hon,
Fransson Thomas,
Pham Cindy C.,
Nelson Silke,
Song Sanghoon,
Britz Alexander,
Messinger Johannes,
Bergmann Uwe,
Alonso-Mori Roberto,
Yachandra Vittal K.,
Kern Jan,
Yano Junko
Publication year - 2019
Publication title -
journal of synchrotron radiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.172
H-Index - 99
ISSN - 1600-5775
DOI - 10.1107/s1600577519007550
Subject(s) - extended x ray absorption fine structure , xanes , x ray absorption spectroscopy , analytical chemistry (journal) , absorption spectroscopy , materials science , synchrotron , spectroscopy , chemistry , optics , physics , chromatography , quantum mechanics
This work has demonstrated that X‐ray absorption spectroscopy (XAS), both Mn XANES and EXAFS, of solutions with millimolar concentrations of metal is possible using the femtosecond X‐ray pulses from XFELs. Mn XAS data were collected using two different sample delivery methods, a Rayleigh jet and a drop‐on‐demand setup, with varying concentrations of Mn. Here, a new method for normalization of XAS spectra based on solvent scattering that is compatible with data collection from a highly variable pulsed source is described. The measured XANES and EXAFS spectra of such dilute solution samples are in good agreement with data collected at synchrotron sources using traditional scanning protocols. The procedures described here will enable XFEL‐based XAS on dilute biological samples, especially metalloproteins, with low sample consumption. Details of the experimental setup and data analysis methods used in this XANES and EXAFS study are presented. This method will also benefit XAS performed at high‐repetition‐rate XFELs such as the European XFEL, LCLS‐II and LCLS‐II‐HE.