Open Access3D digital anatomic angioarchitecture of the mouse brain using synchrotron‐radiation‐based propagation phase‐contrast imaging
Author(s) -
Shi Shupeng,
Zhang Haoran,
Yin Xianzhen,
Wang Zhuolu,
Tang Bin,
Luo Yuebei,
Ding Hui,
Chen Zhuohui,
Cao Yong,
Wang Tiantian,
Xiao Bo,
Zhang Mengqi
Publication year - 2019
Publication title -
journal of synchrotron radiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.172
H-Index - 99
ISSN - 1600-5775
DOI - 10.1107/s160057751900674x
Subject(s) - visualization , neurovascular bundle , computer science , phase contrast imaging , neuroanatomy , contrast (vision) , phase contrast microscopy , biomedical engineering , medicine , anatomy , computer vision , artificial intelligence , physics , optics
Thorough investigation of the three‐dimensional (3D) configuration of the vasculature of mouse brain remains technologically difficult because of its complex anatomical structure. In this study, a systematic analysis is developed to visualize the 3D angioarchitecture of mouse brain at ultrahigh resolution using synchrotron‐radiation‐based propagation phase‐contrast imaging. This method provides detailed restoration of the intricate brain microvascular network in a precise 3D manner. In addition to depicting the delicate 3D arrangements of the vascular network, 3D virtual micro‐endoscopy is also innovatively performed to visualize randomly a selected vessel within the brain for both external 3D micro‐imaging and endoscopic visualization of any targeted microvessels, which improves the understanding of the intrinsic properties of the mouse brain angioarchitecture. Based on these data, hierarchical visualization has been established and a systematic assessment on the 3D configuration of the mouse brain microvascular network has been achieved at high resolution which will aid in advancing the understanding of the role of vasculature in the perspective of structure and function in depth. This holds great promise for wider application in various models of neurovascular diseases.