Open AccessScanning transmission X‐ray microscopy with efficient X‐ray fluorescence detection (STXM‐XRF) for biomedical applications in the soft and tender energy range
Author(s) -
Lühl Lars,
Andrianov Konstantin,
Dierks Hanna,
Haidl Andreas,
Dehlinger Aurelie,
Heine Markus,
Heeren Jörg,
Nisius Thomas,
Wilhein Thomas,
Kanngießer Birgit
Publication year - 2019
Publication title -
journal of synchrotron radiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.172
H-Index - 99
ISSN - 1600-5775
DOI - 10.1107/s1600577518016879
Subject(s) - fluorescence , microscopy , microscope , materials science , optics , x ray fluorescence , characterization (materials science) , fluorescence microscope , detector , analytical chemistry (journal) , nanotechnology , chemistry , physics , chromatography
Scanning transmission X‐ray microscopy, especially in combination with X‐ray fluorescence detection (STXM‐XRF) in the soft X‐ray energy range, is becoming an increasingly important tool for life sciences. Using X‐ray fluorescence detection, the study of biochemical mechanisms becomes accessible. As biological matrices generally have a low fluorescence yield and thus a low fluorescence signal, high detector efficiency ( e.g. large solid angle) is indispensable for avoiding long measurement times and radiation damage. Here, the new AnImaX STXM‐XRF microscope equipped with a large solid angle of detection enabling fast scans and the first proof‐of‐principle measurements on biomedical samples are described. In addition, characterization measurements for future quantitative elemental imaging are presented.