Open AccessImplications of X‐ray beam profiles on qualitative and quantitative synchrotron micro‐focus X‐ray fluorescence microscopy
Author(s) -
Morrell Alexander P.,
Mosselmans J. Frederick W.,
Geraki Kalotina,
Ignatyev Konstantin,
Castillo-Michel Hiram,
Monksfield Peter,
Warfield Adrian T.,
Febbraio Maria,
Roberts Helen M.,
Addison Owen,
Martin Richard A.
Publication year - 2018
Publication title -
journal of synchrotron radiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.172
H-Index - 99
ISSN - 1600-5775
DOI - 10.1107/s160057751801247x
Subject(s) - synchrotron , synchrotron radiation , optics , beam (structure) , microscopy , x ray fluorescence , materials science , microscope , x ray , thresholding , fluorescence , physics , computer science , artificial intelligence , image (mathematics)
Synchrotron radiation X‐ray fluorescence microscopy is frequently used to investigate the spatial distribution of elements within a wide range of samples. Interrogation of heterogeneous samples that contain large concentration ranges has the potential to produce image artefacts due to the profile of the X‐ray beam. The presence of these artefacts and the distribution of flux within the beam profile can significantly affect qualitative and quantitative analyses. Two distinct correction methods have been generated by referencing the beam profile itself or by employing an adaptive‐thresholding procedure. Both methods significantly improve qualitative imaging by removing the artefacts without compromising the low‐intensity features. The beam‐profile correction method improves quantitative results but requires accurate two‐dimensional characterization of the X‐ray beam profile.