Open AccessProtein crystallography beamline (PX‐BL21) at Indus‐2 synchrotron
Author(s) -
Kumar Ashwani,
Ghosh Biplab,
Poswal H. K.,
Pandey K. K.,
Hosur M. V.,
Dwivedi Abhilash,
Makde Ravindra D.,
Sharma Surinder M.
Publication year - 2016
Publication title -
journal of synchrotron radiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.172
H-Index - 99
ISSN - 1600-5775
DOI - 10.1107/s160057751600076x
Subject(s) - beamline , monochromator , synchrotron , goniometer , optics , protein crystallization , diffraction , sample (material) , detector , materials science , beam (structure) , crystallography , physics , chemistry , crystallization , wavelength , thermodynamics
The protein crystallography beamline (PX‐BL21), installed at the 1.5 T bending‐magnet port at the Indian synchrotron (Indus‐2), is now available to users. The beamline can be used for X‐ray diffraction measurements on a single crystal of macromolecules such as proteins, nucleic acids and their complexes. PX‐BL21 has a working energy range of 5–20 keV for accessing the absorption edges of heavy elements commonly used for phasing. A double‐crystal monochromator [Si(111) and Si(220)] and a pair of rhodium‐coated X‐ray mirrors are used for beam monochromatization and manipulation, respectively. This beamline is equipped with a single‐axis goniometer, Rayonix MX225 CCD detector, fluorescence detector, cryogenic sample cooler and automated sample changer. Additional user facilities include a workstation for on‐site data processing and a biochemistry laboratory for sample preparation. In this article the beamline, other facilities and some recent scientific results are briefly described.