Open AccessImperfection and radiation damage in protein crystals studied with coherent radiation
Author(s) -
Nave Colin,
Sutton Geoff,
Evans Gwyndaf,
Owen Robin,
Rau Christoph,
Robinson Ian,
Stuart David Ian
Publication year - 2016
Publication title -
journal of synchrotron radiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.172
H-Index - 99
ISSN - 1600-5775
DOI - 10.1107/s1600577515019700
Subject(s) - diffraction , radiation , coherent diffraction imaging , crystal (programming language) , optics , speckle pattern , materials science , radiation damage , x ray crystallography , physics , computational physics , computer science , phase retrieval , programming language , quantum mechanics , fourier transform
Fringes and speckles occur within diffraction spots when a crystal is illuminated with coherent radiation during X‐ray diffraction. The additional information in these features provides insight into the imperfections in the crystal at the sub‐micrometre scale. In addition, these features can provide more accurate intensity measurements ( e.g. by model‐based profile fitting), detwinning (by distinguishing the various components), phasing (by exploiting sampling of the molecular transform) and refinement (by distinguishing regions with different unit‐cell parameters). In order to exploit these potential benefits, the features due to coherent diffraction have to be recorded and any change due to radiation damage properly modelled. Initial results from recording coherent diffraction at cryotemperatures from polyhedrin crystals of approximately 2 µm in size are described. These measurements allowed information about the type of crystal imperfections to be obtained at the sub‐micrometre level, together with the changes due to radiation damage.