Open AccessMethodological challenges of optical tweezers‐based X‐ray fluorescence imaging of biological model organisms at synchrotron facilities
Author(s) -
Vergucht Eva,
Brans Toon,
Beunis Filip,
Garrevoet Jan,
Bauters Stephen,
De Rijcke Maarten,
Deruytter David,
Janssen Colin,
Riekel Christian,
Burghammer Manfred,
Vincze Laszlo
Publication year - 2015
Publication title -
journal of synchrotron radiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.172
H-Index - 99
ISSN - 1600-5775
DOI - 10.1107/s1600577515009534
Subject(s) - synchrotron radiation , synchrotron , confocal , optical tweezers , optics , fluorescence , x ray fluorescence , materials science , fluorescence lifetime imaging microscopy , nanotechnology , physics
Recently, a radically new synchrotron radiation‐based elemental imaging approach for the analysis of biological model organisms and single cells in their natural in vivo state was introduced. The methodology combines optical tweezers (OT) technology for non‐contact laser‐based sample manipulation with synchrotron radiation confocal X‐ray fluorescence (XRF) microimaging for the first time at ESRF‐ID13. The optical manipulation possibilities and limitations of biological model organisms, the OT setup developments for XRF imaging and the confocal XRF‐related challenges are reported. In general, the applicability of the OT‐based setup is extended with the aim of introducing the OT XRF methodology in all research fields where highly sensitive in vivo multi‐elemental analysis is of relevance at the (sub)micrometre spatial resolution level.