Open AccessEnergy optimization of a regular macromolecular crystallography beamline for ultra‐high‐resolution crystallography
Author(s) -
Rosenbaum Gerd,
Ginell Stephan L.,
Chen Julian C.H.
Publication year - 2015
Publication title -
journal of synchrotron radiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.172
H-Index - 99
ISSN - 1600-5775
DOI - 10.1107/s1600577514022619
Subject(s) - beamline , undulator , synchrotron , photon energy , advanced photon source , synchrotron radiation , photon , resolution (logic) , high energy x rays , optics , range (aeronautics) , materials science , physics , laser , computer science , beam (structure) , artificial intelligence , composite material
A practical method for operating existing undulator synchrotron beamlines at photon energies considerably higher than their standard operating range is described and applied at beamline 19‐ID of the Structural Biology Center at the Advanced Photon Source enabling operation at 30 keV. Adjustments to the undulator spectrum were critical to enhance the 30 keV flux while reducing the lower‐ and higher‐energy harmonic contamination. A Pd‐coated mirror and Al attenuators acted as effective low‐ and high‐bandpass filters. The resulting flux at 30 keV, although significantly lower than with X‐ray optics designed and optimized for this energy, allowed for accurate data collection on crystals of the small protein crambin to 0.38 Å resolution.