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Identification of grown‐in dislocations in protein crystals by digital X‐ray topography
Author(s) -
Suzuki Ryo,
Abe Marina,
Kojima Kenichi,
Tachibana Masaru
Publication year - 2021
Publication title -
journal of applied crystallography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.429
H-Index - 162
ISSN - 1600-5767
DOI - 10.1107/s1600576720015356
Subject(s) - dislocation , burgers vector , crystallography , tetragonal crystal system , bent molecular geometry , materials science , crystal (programming language) , dislocation creep , protein crystallization , x ray , condensed matter physics , optics , physics , chemistry , crystal structure , crystallization , computer science , composite material , thermodynamics , programming language
X‐ray topography is a useful and nondestructive method for direct observation of crystal defects in nearly perfect single crystals. The grown‐in dislocations from the cross‐linked seed crystal in tetragonal hen egg‐white lysozyme crystals were successfully characterized by digital X‐ray topography. Digital X‐ray topographs with various reflections were easily obtained by reconstruction of sequential rocking‐curve images. The Burgers vector of the dislocation is different from those reported previously. Interestingly, one of the dislocations had a bent shape. The preferred direction of the dislocation line was analysed by the estimated dislocation energy based on the dislocation theory. The dislocation energy can be estimated by the dislocation theory even in protein crystals composed of macromolecules.