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SAS‐cam : a program for automatic processing and analysis of small‐angle scattering data
Author(s) -
Wu Hongjin,
Li Yiwen,
Liu Guangfeng,
Liu Haiguang,
Li Na
Publication year - 2020
Publication title -
journal of applied crystallography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.429
H-Index - 162
ISSN - 1600-5767
DOI - 10.1107/s1600576720008985
Subject(s) - beamline , small angle x ray scattering , pipeline (software) , computer science , synchrotron radiation , throughput , synchrotron , visualization , computational science , process (computing) , scattering , computer graphics (images) , beam (structure) , optics , physics , data mining , operating system , wireless
Small‐angle X‐ray scattering (SAXS) is a widely used method for investigating biological macromolecules in structural biology, providing information on macromolecular structures and dynamics in solution. Modern synchrotron SAXS beamlines are characterized as high‐throughput, capable of collecting large volumes of data and thus demanding fast data processing for efficient beamline operations. This article presents a fully automated and high‐throughput SAXS data analysis pipeline, SAS‐cam , primarily based on the SASTBX package. Five modules are included in SAS‐cam , encompassing the data analysis process from data reduction to model interpretation. The model parameters are extracted from SAXS profiles and stored in an HTML summary file, ready for online visualization using a web browser. SAS‐cam can provide the user with the possibility of optimizing experimental parameters based on real‐time feedback and it therefore significantly improves the efficiency of beam time. SAS‐cam is installed on the BioSAXS beamline at the Shanghai Synchrotron Radiation Facility. The source code is available upon request.