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Application of a high‐throughput microcrystal delivery system to serial femtosecond crystallography
Author(s) -
Lee Donghyeon,
Park Sehan,
Lee Keondo,
Kim Jangwoo,
Park Gisu,
Nam Ki Hyun,
Baek Sangwon,
Chung Wan Kyun,
Lee Jong-Lam,
Cho Yunje,
Park Jaehyun
Publication year - 2020
Publication title -
journal of applied crystallography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.429
H-Index - 162
ISSN - 1600-5767
DOI - 10.1107/s1600576720002423
Subject(s) - femtosecond , materials science , macromolecule , diffraction , nanotechnology , computer science , chemistry , laser , optics , physics , biochemistry
Microcrystal delivery methods are pivotal in the use of serial femtosecond crystallography (SFX) to resolve the macromolecular structures of proteins. Here, the development of a novel technique and instruments for efficiently delivering microcrystals for SFX are presented. The new method, which relies on a one‐dimensional fixed‐target system that includes a microcrystal container, consumes an extremely low amount of sample compared with conventional two‐dimensional fixed‐target techniques at ambient temperature. This novel system can deliver soluble microcrystals without highly viscous carrier media and, moreover, can be used as a microcrystal growth device for SFX. Diffraction data collection utilizing this advanced technique along with a real‐time visual servo scan system has been successfully demonstrated for the structure determination of proteinase K microcrystals at 1.85 Å resolution.