Premium
BILMIX : a new approach to restore the size polydispersity and electron density profiles of lipid bilayers from liposomes using small‐angle X‐ray scattering data
Author(s) -
Konarev Petr V.,
Petoukhov Maxim V.,
Dadinova Liubov A.,
Fedorova Natalia V.,
Volynsky Pavel E.,
Svergun Dmitri I.,
Batishchev Oleg V.,
Shtykova Eleonora V.
Publication year - 2020
Publication title -
journal of applied crystallography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.429
H-Index - 162
ISSN - 1600-5767
DOI - 10.1107/s1600576719015656
Subject(s) - small angle x ray scattering , lipid bilayer , scattering , dispersity , bilayer , vesicle , small angle scattering , electron density , chemical physics , liposome , chemistry , membrane , materials science , analytical chemistry (journal) , crystallography , molecular physics , electron , nanotechnology , optics , chromatography , physics , organic chemistry , quantum mechanics , biochemistry
Small‐angle X‐ray scattering (SAXS) is one of the major tools for the study of model membranes, but interpretation of the scattering data remains non‐trivial. Current approaches allow the extraction of some structural parameters and the electron density profile of lipid bilayers. Here it is demonstrated that parametric modelling can be employed to determine the polydispersity of spherical or ellipsoidal vesicles and describe the electron density profile across the lipid bilayer. This approach is implemented in the computer program BILMIX . BILMIX delivers a description of the electron density of a lipid bilayer from SAXS data and simultaneously generates the corresponding size distribution of the unilamellar lipid vesicles.