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A simple adaptation to a protein crystallography station to facilitate difference X‐ray scattering studies
Author(s) -
Sharma Amit,
Berntsen Peter,
Harimoorthy Rajiv,
Appio Roberto,
Sjöhamn Jennie,
Järvå Michael,
Björling Alexander,
Hammarin Greger,
Westenhoff Sebastian,
Brändén Gisela,
Neutze Richard
Publication year - 2019
Publication title -
journal of applied crystallography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.429
H-Index - 162
ISSN - 1600-5767
DOI - 10.1107/s1600576719001900
Subject(s) - small angle x ray scattering , beamline , scattering , crystallography , materials science , optics , infrared , beam (structure) , chemistry , physics
The X‐ray crystallography station I911‐2 at MAXLab II (Lund, Sweden) has been adapted to enable difference small‐ and wide‐angle X‐ray scattering (SAXS/WAXS) data to be recorded. Modifications to the beamline included a customized flow cell, a motorized flow cell holder, a helium cone, a beam stop, a sample stage and a sample delivery system. This setup incorporated external devices such as infrared lasers, LEDs and reaction mixers to induce conformational changes in macromolecules. This platform was evaluated through proof‐of‐principle experiments capturing light‐induced conformational changes in phytochromes. A difference WAXS signature of conformational changes in a plant aquaporin was also demonstrated using caged calcium.