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Neutron diffraction from aligned stacks of lipid bilayers using the WAND instrument
Author(s) -
Marquardt Drew,
Frontzek Matthias D.,
Zhao Yu,
Chakoumakos Bryan C.,
Katsaras John
Publication year - 2018
Publication title -
journal of applied crystallography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.429
H-Index - 162
ISSN - 1600-5767
DOI - 10.1107/s1600576718001243
Subject(s) - neutron diffraction , lipid bilayer , neutron , materials science , analytical chemistry (journal) , chemistry , crystallography , membrane , chromatography , crystal structure , physics , biochemistry , quantum mechanics
Neutron diffraction from aligned stacks of lipid bilayers is examined using the Wide‐Angle Neutron Diffractometer (WAND), located at the High Flux Isotope Reactor, Oak Ridge, Tennessee, USA. Data were collected at different levels of hydration and neutron contrast by varying the relative humidity (RH) and H 2 O/D 2 O ratio from multi‐bilayers of dioleoylphosphatidylcholine and sunflower phosphatidylcholine extract aligned on single‐crystal silicon substrates. This work highlights the capabilites of a newly fabricated sample hydration cell, which allows the lipid bilayers to be hydrated with varying H/D ratios from the RH generated by saturated salt solutions, and also demonstrates WAND's capability as an instrument suitable for the study of aligned lipid multi‐bilayers.