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SEC‐SANS: size exclusion chromatography combined in situ with small‐angle neutron scattering
Author(s) -
Jordan Ashley,
Jacques Mark,
Merrick Catherine,
Devos Juliette,
Forsyth V. Trevor,
Porcar Lionel,
Martel Anne
Publication year - 2016
Publication title -
journal of applied crystallography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.429
H-Index - 162
ISSN - 1600-5767
DOI - 10.1107/s1600576716016514
Subject(s) - neutron scattering , small angle neutron scattering , scattering , small angle scattering , size exclusion chromatography , neutron , in situ , chemistry , physics , optics , nuclear physics , nuclear magnetic resonance , enzyme , organic chemistry
The first implementation and use of an in situ size exclusion chromatography (SEC) system on a small‐angle neutron scattering instrument (SANS) is described. The possibility of deploying such a system for biological solution scattering at the Institut Laue–Langevin (ILL) has arisen from the fact that current day SANS instruments at ILL now allow datasets to be acquired using small sample volumes with exposure times that are often shorter than a minute. This capability is of particular importance for the study of unstable biological macromolecules where aggregation or denaturation issues are a major problem. The first use of SEC‐SANS on ILL's instrument D22 is described for a variety of proteins including one particularly aggregation‐prone system.