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Reconstructing three‐dimensional helical structure with an X‐ray free electron laser
Author(s) -
Uddin M.
Publication year - 2016
Publication title -
journal of applied crystallography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.429
H-Index - 162
ISSN - 1600-5767
DOI - 10.1107/s1600576716000303
Subject(s) - diffraction , free electron laser , optics , laser , scattering , physics , electron diffraction , crystallography , computational physics , chemistry
Recovery of three‐dimensional structure from single‐particle X‐ray scattering of completely randomly oriented diffraction patterns as predicted a few decades ago has been realized owing to the advent of the new emerging X‐ray free electron laser (XFEL) technology. Since the world's first XFEL started operation in June 2009 at SLAC National Laboratory at Stanford, the first few experiments have been conducted on larger objects such as viruses. Many of the important structures of nature such as helical viruses or DNA consist of helical repetition of biological subunits. Hence development of a method for reconstructing helical structure from collected XFEL data has been a top research priority. This work describes the development of a method for solving helical structures such as tobacco mosaic virus from a set of randomly oriented simulated diffraction patterns exploiting the symmetry and Fourier space constraint of the diffraction volume.