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Scattering from phase‐separated vesicles. I. An analytical form factor for multiple static domains
Author(s) -
Heberle Frederick A.,
Anghel Vinicius N.P.,
Katsaras John
Publication year - 2015
Publication title -
journal of applied crystallography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.429
H-Index - 162
ISSN - 1600-5767
DOI - 10.1107/s160057671501362x
Subject(s) - scattering , small angle scattering , monte carlo method , truncation (statistics) , neutron scattering , biological small angle scattering , structure factor , context (archaeology) , small angle neutron scattering , statistical physics , vesicle , domain (mathematical analysis) , phase (matter) , series (stratigraphy) , physics , computational physics , chemistry , optics , crystallography , mathematics , mathematical analysis , statistics , quantum mechanics , paleontology , biochemistry , membrane , biology
This is the first in a series of papers considering elastic scattering from laterally heterogeneous lipid vesicles containing multiple domains. Unique among biophysical tools, small‐angle neutron scattering can in principle give detailed information about the size, shape and spatial arrangement of domains. A general theory for scattering from laterally heterogeneous vesicles is presented, and the analytical form factor for static domains with arbitrary spatial configuration is derived, including a simplification for uniformly sized round domains. The validity of the model, including series truncation effects, is assessed by comparison with simulated data obtained from a Monte Carlo method. Several aspects of the analytical solution for scattering intensity are discussed in the context of small‐angle neutron scattering data, including the effect of varying domain size and number, as well as solvent contrast. The analysis indicates that effects of domain formation are most pronounced when the vesicle's average scattering length density matches that of the surrounding solvent.