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A suite of software for processing MicroED data of extremely small protein crystals
Author(s) -
Iadanza Matthew G.,
Gonen Tamir
Publication year - 2014
Publication title -
journal of applied crystallography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.429
H-Index - 162
ISSN - 1600-5767
DOI - 10.1107/s1600576714008073
Subject(s) - suite , software suite , python (programming language) , software , computer science , background subtraction , computational science , data processing , computer graphics (images) , artificial intelligence , database , programming language , pixel , archaeology , history
Electron diffraction of extremely small three‐dimensional crystals (MicroED) allows for structure determination from crystals orders of magnitude smaller than those used for X‐ray crystallography. MicroED patterns, which are collected in a transmission electron microscope, were initially not amenable to indexing and intensity extraction by standard software, which necessitated the development of a suite of programs for data processing. The MicroED suite was developed to accomplish the tasks of unit‐cell determination, indexing, background subtraction, intensity measurement and merging, resulting in data that can be carried forward to molecular replacement and structure determination. This ad hoc solution has been modified for more general use to provide a means for processing MicroED data until the technique can be fully implemented into existing crystallographic software packages. The suite is written in Python and the source code is available under a GNU General Public License.