Open AccessX‐ray Imaging and Microspectroscopy of Plants and Fungi
Author(s) -
Yun W.,
Pratt S. T.,
Miller R. M.,
Cai Z.,
Hunter D. B.,
Jarstfer A. G.,
Kemner K. M.,
Lai B.,
Lee H.R.,
Legnini D. G.,
Rodrigues W.,
Smith C. I.
Publication year - 1998
Publication title -
journal of synchrotron radiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.172
H-Index - 99
ISSN - 1600-5775
DOI - 10.1107/s0909049598007225
Subject(s) - microprobe , synchrotron radiation , xanes , synchrotron , x ray fluorescence , resolution (logic) , analytical chemistry (journal) , image resolution , x ray , microscopy , fluorescence , chemistry , elemental analysis , absorption (acoustics) , optics , materials science , spectral line , mineralogy , physics , environmental chemistry , organic chemistry , astronomy , artificial intelligence , computer science
X‐ray fluorescence microscopy and microspectroscopy with micrometre spatial resolution and unprecedented capabilities for the study of biological and environmental samples are reported. These new capabilities are a result of both the combination of high‐brilliance synchrotron radiation and high‐performance X‐ray microfocusing optics and the intrinsic advantages of X‐rays for elemental mapping and chemical‐state imaging. In this paper, these capabilities are illustrated by experimental results on hard X‐ray phase‐contrast imaging, X‐ray fluorescence (XRF) imaging and microspectroscopy of mycorrhizal plant roots and fungi in their natural hydrated state. The XRF microprobe is demonstrated by the simultaneous mapping of the elemental distributions of P, S, K, Ca, Mn, Fe, Ni, Cu and Zn with a spatial resolution of approximately 1 × 3 µm and with an elemental sensitivity of approximately 500 p.p.b. Microspectroscopy with the same spatial resolution is demonstrated by recording near‐edge X‐ray absorption (XANES) spectra of Mn at a concentration of approximately 3 p.p.m.