Open AccessHigh‐Pressure Solution X‐ray Scattering of Protein Using a Hydrostatic Cell with Diamond Windows
Author(s) -
Kato Minoru,
Fujisawa Tetsuro
Publication year - 1998
Publication title -
journal of synchrotron radiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.172
H-Index - 99
ISSN - 1600-5775
DOI - 10.1107/s0909049598000788
Subject(s) - radius of gyration , lysozyme , diamond anvil cell , hydrostatic pressure , scattering , small angle x ray scattering , radius , materials science , high pressure , diamond , hydrostatic equilibrium , analytical chemistry (journal) , crystallography , chemistry , optics , thermodynamics , chromatography , physics , composite material , polymer , biochemistry , computer security , computer science , quantum mechanics
A hydrostatic high‐pressure cell (maximum pressure 700 MPa) with synthetic diamond windows is applied to measure small‐angle X‐ray scattering of a protein at high pressure. Use of the present cell allows an accurate solvent background correction, providing quantitative analyses. The performance of the present cell for X‐ray scattering is shown by using lysozyme as a sample solution. From the Guinier plot, values of the radius of gyration of lysozyme are evaluated to be 15.31 ± 0.09 Å at 1 atm (0.10 MPa) and 14.80 ± 0.15 Å at 400 MPa.