Open AccessStudies on intracellular structures of COS cells by X‐ray microscopy
Author(s) -
Yamamoto Akitsugu,
Masaki Ryuichi,
Guttmann Peter,
Schmahl Günter,
Kihara Hiroshi
Publication year - 1998
Publication title -
journal of synchrotron radiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.172
H-Index - 99
ISSN - 1600-5775
DOI - 10.1107/s0909049597015823
Subject(s) - nucleolus , nucleus , electron microscope , transmission electron microscopy , chemistry , microscopy , nucleoplasm , crystallography , x ray , biophysics , ultrastructure , biology , microbiology and biotechnology , materials science , anatomy , physics , nanotechnology , optics
COS‐7 cells, fixed with glutaraldehyde, were studied using the transmission X‐ray microscope at the electron storage ring BESSY, Berlin. The border of the cell, the nucleus, nucleoli and mitochondria of the cells were clearly visualized with the X‐ray microscope. In addition, we found many X‐ray dense granules preferentially located around the nucleus. Electron microscopy showed that numerous multivesicular bodies, whose structures belong to the endosome–lysosomal system, were present around the nucleus. The size and localization patterns of the X‐ray dense granules were quite similar to those of multivesicular bodies. These results strongly suggest that the X‐ray dense granules are multivesicular bodies.