Open AccessQuick Fluorescence‐EXAFS: an Improved Method for Collection of Conventional XAFS Data, an Improved Method for Collection of Conventional XAFS Data and for Studying Reaction Intermediates in Dilute Systems
Author(s) -
Murphy L. M.,
Dobson B. R.,
Neu M.,
Ramsdale C. A.,
Strange R. W.,
Hasnain S. S.
Publication year - 1995
Publication title -
journal of synchrotron radiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.172
H-Index - 99
ISSN - 1600-5775
DOI - 10.1107/s0909049594010861
Subject(s) - extended x ray absorption fine structure , x ray absorption fine structure , monochromator , fluorescence , chemistry , absorption (acoustics) , analytical chemistry (journal) , materials science , optics , absorption spectroscopy , optoelectronics , physics , spectroscopy , chromatography , wavelength , quantum mechanics , composite material
The quick EXAFS (QuEXAFS) technique provides an alternative way of recording X‐ray absorption fine‐structure (XAFS) data where the scan time is reduced by moving the monochromator at a constant angular speed and recording the data `on the fly'. Results are presented to show that the use of fluorescence detection with QuEXAFS is eminently suitable for studying reactions in dilute systems such as metalloproteins at a sub‐minute time scale. In addition, we show that the fluorescence–QuEXAFS technique can reduce the overall time for normal data collection by some 50% compared with conventional step‐by‐step scanning EXAFS using the same optical system, thus reducing the total X‐ray exposures of the samples. The use of X‐rays for studying in situ redox reactions is demonstrated.