Open AccessStructure of liposome encapsulating proteins characterized by X‐ray scattering and shell‐modeling
Author(s) -
Hirai Mitsuhiro,
Kimura Ryota,
Takeuchi Kazuki,
Hagiwara Yoshihiko,
KawaiHirai Rika,
Ohta Noboru,
Igarashi Noriyuki,
Shimuzu Nobutaka
Publication year - 2013
Publication title -
journal of synchrotron radiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.172
H-Index - 99
ISSN - 1600-5775
DOI - 10.1107/s0909049513020827
Subject(s) - liposome , extrusion , lipid bilayer , drug delivery , materials science , chemistry , biophysics , nanotechnology , membrane , biochemistry , composite material , biology
Lipid liposomes are promising drug delivery systems because they have superior curative effects owing to their high adaptability to a living body. Lipid liposomes encapsulating proteins were constructed and the structures examined using synchrotron radiation small‐ and wide‐angle X‐ray scattering (SR‐SWAXS). The liposomes were prepared by a sequential combination of natural swelling, ultrasonic dispersion, freeze‐throw, extrusion and spin‐filtration. The liposomes were composed of acidic glycosphingolipid (ganglioside), cholesterol and phospholipids. By using shell‐modeling methods, the asymmetric bilayer structure of the liposome and the encapsulation efficiency of proteins were determined. As well as other analytical techniques, SR‐SWAXS and shell‐modeling methods are shown to be a powerful tool for characterizing in situ structures of lipid liposomes as an important candidate of drug delivery systems.